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CoaChrom®
α1-Antitrypsin

Produkt Nr.

COA0016

VE

200 Tests

Beschreibung

This kit is designed for the assay of α1-antitrypsin (α1-AT, α1-proteinase inhibitor) in human plasma. Plasma is diluted in buffer containing methylamine, which inactivates α2-macroglobulin. Excess trypsin is added and during an incubation period, it complexes with α1-AT. The remaining free trypsin is measured by its ability to cleave a chromogenic peptide substrate. The release of p-nitroaniline (pNA) from the substrate is measured at 405nm, and the log absorbance is inversely proportional to the plasma α1-AT concentration.

Verwendungszweck

Assay proposed for the measurement of α1-antitrypsin (α1-AT, α1-proteinase inhibitor) in human plasma. For Research Use only.

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